BIOLUMINESCENT BEHAVIOR IN RENILLA. I. COLONIAL RESPONSES

Bioluminescent indicators for Ca2+ based on split Renilla luciferase complementation in living cells.

Genetically encoded bioluminescent indicators for intracellular Ca2+ are described here with CaM-M13 interaction-induced complementation of split Renilla luciferase. The Ca2+-induced interaction between CaM and M13 leads to complementation of the N- and C-terminal halves of split Renilla luciferase in living cells. This intramolecular interaction results in the spontaneous and simultaneous emis...

Colonial Responses of Hydroid Polyps

I-33: Oxidative Stress Responses in EarlyPregnancy

Background: Survival of the conceptus is dependent on continuous progesterone signaling in the maternal decidua but how this is achieved under conditions of oxidative stress that characterize early pregnancy is unknown. Materials and Methods: Laboratory-based analysis of endometrial biopsies and primary endometrial cultures. Results: Using primary cultures, we show that modest levels of reactiv...

A fine structure study of the anthocodium in Renilla mulleri. Evidence for the existence of a bioluminescent organelle, the luminelle

A fine structure study of the anthocodium of the sea pansy, Renilla mülleri, was undertaken. The anthocodium, a known site of bioluminescence, was selected in order to determine whether a structural entity could be found which would satisfy the biochemical and physiological features associated with the known sites of bioluminescence in this animal. These sites, termed lumisomes, have previously...

Kinetics of the Bioluminescent Reaction in Cypridina. I

Until the present time it has been proven impossible to isolate as pure chemical individuals any of the reactants concerned in the production of light by organisms. The work of Dubois 1 and Harvey has, however, indicated the general nature of such reactions as consisting in the oxidation of a substrate, luciferin, catalyzed by an enzyme, luciferase. Harvey~ believes that luciferin should be pla...